In order to use the process detailed in this document, you need a sterile and viable Psilocybe cubensis spore syringe. In order to grow subsequent generations of fungus you can generate your own with the mushrooms that you harvest.
There are many varieties of Psilocybe cubensis, you can compare differences and choose the type(s) are most appealing to your preferences. Don't fuss too much, any Psilocybe cubensis variety will work.
US residents should order syringes or prints from sellers inside the US, to avoid customs problems. Residents of the EU should order from within the EU. Many countries are very strict about buying and selling mushroom spores or prints.
Once you have grown some mushrooms, you can learn how to generate your own spore syringes and spore prints so that you have them whenever you need one. If you produce your own, you can eliminate your dependence on suppliers.
There is no guarantee that present vendors of Psilocybe cubensis spore syringes will be in business tomorrow. Learning how to create your own will ensure the ability to raise future crops if there is no option of obtaining a spore syringe from someone else.
Sterility is key. If your spore print is contaminated or you introduce contamination into the spore syringe, you will have difficulty later in the process.
Ideally, there should be no fans blowing or drafts of air. You should clean the area where you will be working carefully and make sure that everything is sterilized. This idea is from AN165023@anon.penet.fi.
Drill a single small hole, about 1/8 of an inch, in the cap of a wide mouth jar. Cover the nail hole with a piece of electrical tape. A 1/2 pint canning jar similar to that used for the cultures is fine. Sterilize both the jar and the lid.
Place the harvested cap in the sterilized, wide mouth jar. Seal the jar by placing the lid on the top, and wait until a spore print has been generated on the bottom of the glass. Open the jar and remove the mushroom cap.
Add 3/4 cup of sterilized water. Seal the top of the jar with the lid and shake the jar. The spores need to be mixed well into the water. This procedure eliminates the need to transfer spores from a print to some container to make the solution.
The spore print is generated inside the container and the only extra step is adding water. It also has the benefit of making a large amount of solution that is easy to use.
Now, any time you need inoculant, you can shake the jar and peel the tape back to expose the nail hole in the lid. Simply insert the needle of the syringe into the inoculant and pull some into the syringe.
The syringe needs to be sterile or you risk contaminating the entire jar of solution. The solution will keep longer if you refrigerate it.
You still should keep some spore prints on paper because it is possible to contaminate the entire jar of inoculant if you make a mistake.
Once you have a mature mushroom, you are in a position to make a spore print and use it to continue cultivation of mushrooms. The cap should be harvested when the mushroom cap has become flat or is starting to invert.
Sterility is key (adaptation-25). Be careful not to do anything that will compromise the sterility of your spore print. The typical procedure is to cut the stalk off of a mature mushroom very close to the cap. A sterilized knife or razor blade is used to do this.
The cap is then laid on a sterile piece of tissue paper or card stock and a small glass set over it. The glass is needed for two reasons. First, it keeps the spore print insulated from airborne contaminants. Secondly, it helps keep the humidity high so the mushroom cap can continue to live and drop its spores.
One note of caution. Some humidity usually needs to be allowed to escape. You want the environment inside the glass to be slightly less humid than the environment in which the mushroom was grown.
If you have problems getting a cap to drop its spores, try using a piece of paper for the print that fits entirely inside the glass and spreading out a wash cloth flat on the table. Let the edge of the glass seal to the wash cloth instead of the paper. This will usually allow enough humidity to escape to cause the cap to drop spores.
If everything goes well, after a day or two the cap will drop its spores (adaptation-26). There will be a purple-brown dust underneath the cap.
Eye glass lens paper is a good source of sterile tissue paper. A box of waxy tissue paper that deli's use to pick up donuts and rolls is another excellent source of sterile tissue paper.
Card stock (such as a recipe card) is a bit easier to use later when you want to prepare a spore syringe, but you have to expend the extra effort up front to sterilize it. To use card stock, place in a 425 degree fahrenheit oven for 15 minutes. Let it cool with a glass over it before you place the cap on it.
Once you have a spore print on the paper, remove the glass and cap. Fold the spore print in half and seal the edges so air can not get in. A piece of scotch tape on each side will do nicely. The spores will stay viable for 18 months if they are kept in a cool, dry and dark spot.
If you place a small amount of desiccant in the bottom of a film container and place a cotton ball on top of the desiccant, you have an ideal container to keep the spore print.
The cotton ball will keep the desiccant from touching the spore print. Seal the spore print in the canister and place the canister in your refrigerator until you are ready to use it.
Note that if you want to be self sufficient, it is a good idea to have multiple spore prints and store them separately. You just never know when you are going to be surprised with a massive contamination problem or thermal death.
The safest thing to do is have a couple viable spore prints so it is easier to recover from disaster. A spore print is typically viable for about a year if it is stored in a cool, dark location. As a print ages, germination gets slower and this process becomes more prone to contamination.
--- Coffee mug.
--- Shot glass.
--- Spore print.
--- Regular glass.
--- Microwave oven.
--- Cigarette lighter.
--- 10 cc syringe (description).
--- X-acto knife or sharp steak knife.
Several things need to be accomplished. First, we need to sterilize a shot glass to easily mix the spore solution and we need to sterilize a syringe to hold the solution.
We also need some sterile water in which to suspend the spores. The following procedure will accomplish all of this. Fill a coffee mug with water and place a shot glass inside the coffee mug.
Make sure the shot glass is completely submerged. Place the coffee mug in the microwave oven and get the water to a full boil for 10 minutes. It does not need to be a violent boil.
Adjust the heat level of the microwave oven to keep too much water from being lost if necessary. Remove the shot glass and empty the excess water out of the shot glass.
Place another glass over the shot glass. This will keep airborne contaminants from settling in the shot glass while you wait for the shot glass and water in the spore syringe to cool.
Fill the syringe with hot water from the mug. Eject the hot water and repeat several times. This will insure the inside of the syringe and the needle are clean and sterile. This is especially important if you are using a syringe from a previous crop.
When the needle is inserted into the substrate, it is possible to get nutrients up inside the needle and for contamination to grow. The last time you fill the syringe with hot water, do not purge it. Let it sit in the syringe until it is cool.
This is useful for two reasons. First, the continued heat from the water can still work to eliminate any remaining contaminants. Secondly, once the water is cool it can be used as the sterile water needed to fill the syringe.
Make certain that nothing touches the needle of the syringe. The psilocybe mushroom spores will be killed if they come in contact with anything too hot. You need to wait until the shot glass and spore syringe are at room temperature.
When it is safe to proceed, use the cigarette lighter to flame sterilize the X-Acto knife and the needle of the syringe. Let the blade of the knife cool, but make sure it does not touch anything.
When it is cool, carefully open the spore print and scrape a fleck of spores into the shot glass. A fleck 1/4 inch by 1/4 inch is more than sufficient for a 10 cc. spore syringe.
Use the needle of the syringe to stir the spores into a few drops of water. Usually, there will be a few drops left over in the shot glass from when you emptied it. Otherwise, you can get the drops from the syringe.
Stir the fleck of spores until they are well broken up and 'dissolved' into the water. Purge the water out of the syringe into the shot glass. Pull the water back into the syringe, being sure to suck the spores in also.
Do this once or twice more to make sure the spores are well mixed in the spore syringe. Often, it takes several tries to get the spores fully broken apart and well mixed.
If the spores in the print have been dried and are not fresh, it is best to wait six hours to use the spore syringe. The spores need to rehydrate. If you're in a hurry, the spores can still rehydrate in the culture jars.
Grow Magic Mushrooms Index
--- Growing Cycle
--- Drying, Preserving
--- Dosage And Ingesting
--- Getting A Spore Syringe
--- Getting Stuff
--- Growing In Bulk
--- List Of Adaptations